RNA Sequencing
RNA sequencing (RNA-seq) is a high-throughput sequencing technique that allows researchers to study gene expression and transcriptome profiling at a whole-genome level. RNA-seq involves the isolation of RNA molecules from a biological sample, conversion of RNA to cDNA, and sequencing of the cDNA library using next-generation sequencing technologies. The resulting data can be used to identify differentially expressed genes, alternative splicing events, novel transcripts, and non-coding RNAs. RNA-seq has revolutionized the field of transcriptomics and has become a powerful tool for understanding gene regulation, developmental biology, disease mechanisms, and drug discovery.
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